USB4000-FL-450 and USB4000-FL-395 Spectrofluorometers
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The USB4000-FL Spectrofluorometers are preconfigured, fully integrated fluorescence spectrometers. The USB4000-FL-450 comes with a USB-LS-450 direct-attach light source while the USB4000-FL-395 comes with an LS-395 LED Module. These spectrofluorometers can be used for general fluorescence measurements.
The USB4000-FL-450 is available for $3523. The USB4000-FL-395 is available for $3523
The USB4000-FL spectrofluorometers cover the 360-1100 nm wavelength range. They feature a 200 µm entrance aperture and an L4 Detector Collection Lens to increase light-collection efficiency and reduce stray light. |
The direct-attach USB-LS-450 Pulsed Blue LED Light Source is designed as an excitation source that produces either pulsed or continuous output centered at 470 nm for fluorescence measurements.
The direct-attach USB-LS-395 produces either pulsed or continuous output centered at 395 nm.
Programmable Microcontroller
The USB4000-FL Spectrofluorometers' onboard programmable microcontroller provides you with considerable flexibility in controlling the spectrometer and accessories. Through a 22-pin connector, you can implement all operating parameters in the software: control light sources, create processes, and retrieve information on external objects. You have access to 8 user-programmable digital Inputs/Outputs for interfacing to other equipment. (Contact an Applications Scientist regarding what you'll need to program the GPIOs.)
Optimal Performance
The USB4000-FL Spectrofluormeter provides you with an affordable, high-performance 3648-element linear CCD-array detector with an optical bench that's small enough to fit into the palm of your hand. The USB4000-FL works the same way as other Ocean Optics spectrometers; it accepts light energy transmitted through single-strand optical fiber and disperses it via a fixed grating across the linear CCD array detector, which is responsive from 200-1100 nm.
Plug and Play Operation
The USB4000-FL spectrofluorometers interface to a PC, PLC or other embedded controllers via USB 2.0 or RS-232 serial port. When using the serial port, the USB4000-FL requires a single 5-volt power supply (not included). Data unique to each spectrometer is programmed into a memory chip on the USB4000-FL; our spectrometer operating software reads these values for easy setup and hot swapping among PCs.
Specifications
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Physical |
| Spectrometer Dimensions: |
89.1 mm x 63.3 mm x 34.4 mm |
| Weight: |
190 grams |
| Detector Specifications |
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Detector: |
Toshiba TCD1304AP Linear CCD array |
| Detector range: |
200-1100 nm |
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Pixels: |
3648 pixels |
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Pixel size: |
8 μm x 200 μm |
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Pixel well depth: |
100,000 electrons |
| Signal-to-noise ratio: |
300:1 (at full signal) |
| A/D resolution: |
16 bit |
| Dark noise: |
50 RMS counts |
| Corrected linearity: |
>99.8% |
| Sensitivity: |
130 photons/count at 400 nm; 60 photons/count at 600 nm |
| Optical Bench |
| Design: |
f/4, Asymmetrical crossed Czerny-Turner |
| Focal length: |
42 mm input; 68 mm output |
| Entrance aperture: |
200 µm wide slit |
| Grating: |
Grating #3 -- groove density 600 lines per millimeter, 360-1000 nm, 500 nm blaze |
| Detector collection lens: |
Yes, L4 |
| Fiber optic connector: |
SMA 905 to 0.22 numerical aperture single-strand optical fiber |
| Spectroscopic |
| Wavelength range: |
360-1000 nm |
| Optical resolution: |
~10.0 nm FWHM |
| Signal-to-noise ratio: |
300:1 (at full signal) |
| A/D resolution: |
16 bit |
| Dark noise: |
50 RMS counts |
| Dynamic range: |
2 x 108 (system), 1300:1 for a single acquisition |
| Stray light: |
<0.05% at 600 nm; 0.10% at 435 nm |
| Electronics |
| Power consumption: |
250 mA @ 5 VDC |
| Data transfer speed: |
Full scans to memory every 5 ms with USB 2.0 port |
| Inputs/Outputs: |
Yes, 8 onboard digital user-programmable GPIOs |
| Breakout box compatible: |
Yes, with the USB-ADP-BB adapter |
| Trigger modes: |
4 modes |
| Strobe functions: |
Yes |
| Connector: |
22-pin connector |
| Computer |
| Operating systems: |
Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port |
| Computer interfaces: |
USB 2.0 @ 480 Mbps (USB1.1 compatible); RS-232 (2-wire) @ 115.2 K baud |
| Peripheral interfaces: |
SPI (3-wire); I2C inter-integrated circuit |
USB-LS-450 and USB-LS-395 LED Module
The LED modules connect to the USB4000-FL Spectrometer via a 10-pin connector on the front of the spectrometer. The spectrometer provides power to the LED and also enables synchronization functions and I2C communications.
In addition, the USB-LS-450 and USB-LS-395 feature a built-in, 24-bit analog-to-digital converter with an analog front end that is configured for a 100 ohm platinum RTD (resistance temperature device). This is particularly useful when configured as part of a Fiber Optic Oxygen Sensor. In fact, the unit has on-board memory to store temperature calibration coefficients and oxygen calibration coefficients.
The LED modules can provide 4-20 mA output via a daughter card, making it especially useful for process applications.
The USB-LS-450 wavelength range is 460-490 nm. The USB-LS-395 wavelength range is 380-410 nm.
User's Tip: The LED module boards allow for software-controlled switching between pulsed and CW operation of the LED.
USB-LS-450 Spectral Output
LED Module Specifications
| Criteria |
Specification |
| Power output: |
60 µW into a 600 µm optical fiber |
| LED drive current: |
20 mA +/-150 mA |
| Maximum modulation frequency: |
1 kHz |
| 0.5% stability time: |
less than 1 minute |
| Temperature-dependent drift: |
+0.1%/ºC |
Temperature Measurement Specifications
| Criteria |
Specification |
| Precision: |
better than 0.1 °C |
| Accuracy: |
better than 0.5 °C |
| Maximum data rate: |
15 samples/second |
Power Requirements
| Criteria |
Specification |
| Input voltage range: |
3.0-8.0 volts |
| Quiescent range: |
~20 mA |
| Current with light on: |
~20 mA |
Ordering Information
| Item |
Description |
Price |
| USB4000-FL-450 |
Fully integrated spectrofluorometer features a USB4000-FL Spectrometer and a USB-LS-450 LED Module |
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| USB-LS-450 |
LED drive module for use with USB4000 Spectrometers |
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| USB-LS-450-TP |
Platinum RTD-type temperature probe for USB-LS-450 |
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| USB-LS-450-4/20 |
4-20 mA output module for USB-LS-450 |
Call for Price |
| USB4000-FL-395 |
Fully integrated spectrofluorometer features a USB4000-FL Spectrometer and a USB-LS-395 LED Module |
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| USB-LS-395 |
LED drive module for use with USB4000 Spectrometers |
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| USB4000-FL |
USB Spectrometer (360-1000 nm) |
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